De Novo Leaf Transcriptome Assembly and Metagenomic Studies of Coast Live Oak (Quercus agrifolia)

De Novo Leaf Transcriptome Assembly and Metagenomic Studies of Coast Live Oak (Quercus agrifolia)

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Coast Live Oak (Quercus agrifolia) is a native keystone hardwood species of the California coastal and semi-arid forest environment.Q.agrifolia is threatened by pathogens such as the oomycete click here Phytophthora ramorum, which is known to cause Sudden Oak Death in environments from Southern California to Oregon.This study considers oaks and their rootzone microbes recovering from moderate and low-intensity fires in rapid succession, compared to high- and low-intensity fires with a large time gap between them.

cDNA libraries from nine oak leaf tissue samples were sequenced on DNBseq.Soil samples were sent out for shotgun metagenomics and for 16S community profiling.The de novo Q.agrifolia assembly yielded 521,817 transcripts with an average length of 805.

2 bp.Among identified DEGs (differentially expressed genes) between the trail areas, several candidate genes were identified including shikimate dehydrogenase and myrcene synthase.The MegaBLAST results showed a high degree of similarity to WGS sequences from Q.agrifolia that had been previously annotated in other closely related Quercus species.

There was a differential abundance of microbial genera associated with the different burn areas, including Pedobacter, Filimonas, Cohnella, and Sorangium.The data click here embody the first Q.agrifolia transcriptome that with further development could be used to screen oak seedlings for resistance; beneficial microbial populations have been identified that are associated with fire recovery under varied conditions.